Identification and functional characterization of novel feline cytochrome P450 2A.
作者： Gaku Okamatsu , Tetsuya Komatsu , Akira Kubota , Takenori Onaga , Tsuyoshi Uchide
DOI: 10.3109/00498254.2014.998322
关键词:
摘要: 1. Cytochrome P450s are the major metabolizing enzymes for xenobiotics in humans and other mammals. Although domestic cat Felis catus, an obligate carnivore, is most common companion animal, properties of cytochrome P450 subfamilies largely unknown. 2. We newly identified feline CYP2A13, which consists 494 deduced amino acids, showing highest identity to CYP2As dogs, followed by those pigs, cattle humans. 3. The CYP2A13 transcript protein were expressed almost exclusively liver without particular sex-dependent differences. 4. heterogeneously Escherichia coli showed metabolic activity similar human canine coumarin, 7-ethoxycoumarin nicotine. 5. results indicate importance systemic metabolism cats.
informahealthcare.com参考文章(37)
Hiroshi Iwata, Ken-ichi Fujita, Hirotaka Kushida, Akihiro Suzuki, Yuko Konno, Katsunori Nakamura, Akiharu Fujino, Tetsuya Kamataki, High Catalytic Activity of Human Cytochrome P450 Co-expressed with Human NADPH-Cytochrome P450 Reductase in Escherichia coli Biochemical Pharmacology. ,vol. 55, pp. 1315- 1325 ,(1998) , 10.1016/S0006-2952(97)00643-6
N TANAKA, R SHINKYO, T SAKAKI, M KASAMASTU, S IMAOKA, Y FUNAE, H YOKOTA, Cytochrome P450 2E polymorphism in feline liver. Biochimica et Biophysica Acta. ,vol. 1726, pp. 194- 205 ,(2005) , 10.1016/J.BBAGEN.2005.08.004
Shotaro Uehara, Norie Murayama, Hiroshi Yamazaki, Yasuhiro Uno, A novel CYP2A26 identified in cynomolgus monkey liver metabolizes coumarin Xenobiotica. ,vol. 40, pp. 621- 629 ,(2010) , 10.3109/00498254.2010.501118
Songhee Han, Seunghye Choi, Young-Jin Chun, Chul-Ho Yun, Chang Hoon Lee, Hee Jung Shin, Han Sung Na, Myeon Woo Chung, Donghak Kim, None, Functional Characterization of Allelic Variants of Polymorphic Human Cytochrome P450 2A6 (CYP2A6*5, *7, *8, *18, *19, and *35) Biological & Pharmaceutical Bulletin. ,vol. 35, pp. 394- 399 ,(2012) , 10.1248/BPB.35.394
Xiao-Yang He, Lili Tang, Shou-Lin Wang, Qing-Song Cai, Jia-Sheng Wang, Jun-Yan Hong, Efficient activation of aflatoxin B1 by cytochrome P450 2A13, an enzyme predominantly expressed in human respiratory tract. International Journal of Cancer. ,vol. 118, pp. 2665- 2671 ,(2006) , 10.1002/IJC.21665
Robin Pearce, Denise Greenway, Andrew Parkinson, Species differences and interindividual variation in liver microsomal cytochrome P450 2A enzymes: Effects on coumarin, dicumarol, and testosterone oxidation Archives of Biochemistry and Biophysics. ,vol. 298, pp. 211- 225 ,(1992) , 10.1016/0003-9861(92)90115-D
Marcella Martignoni, Geny M M Groothuis, Ruben de Kanter, Species differences between mouse, rat, dog, monkey and human CYP-mediated drug metabolism, inhibition and induction. Expert Opinion on Drug Metabolism & Toxicology. ,vol. 2, pp. 875- 894 ,(2006) , 10.1517/17425255.2.6.875
J. J. P. Bogaards, M. Bertrand, P. Jackson, M. J. Oudshoorn, R. J. Weaver, P. J. Van Bladeren, B. Walther, Determining the best animal model for human cytochrome P450 activities: a comparison of mouse, rat, rabbit, dog, micropig, monkey and man. Xenobiotica. ,vol. 30, pp. 1131- 1152 ,(2000) , 10.1080/00498250010021684
M. Teresa Donato, Nuria Jiménez, José V. Castell, M. José Gómez-Lechón, FLUORESCENCE-BASED ASSAYS FOR SCREENING NINE CYTOCHROME P450 (P450) ACTIVITIES IN INTACT CELLS EXPRESSING INDIVIDUAL HUMAN P450 ENZYMES Drug Metabolism and Disposition. ,vol. 32, pp. 699- 706 ,(2004) , 10.1124/DMD.32.7.699
Go Kito, Tetsuya Kamataki, Ryoichi Nagata, Yasuhiro Uno, Hideki Fujino, CYP2C76, a novel cytochrome P450 in cynomolgus monkey, is a major CYP2C in liver, metabolizing tolbutamide and testosterone. Molecular Pharmacology. ,vol. 70, pp. 477- 486 ,(2006) , 10.1124/MOL.106.022673