Identification and Analysis of the Tegument Protein and Excretory-Secretory Products of the Carcinogenic Liver Fluke Clonorchis sinensis.

摘要: Liver fluke proteins, including excretory-secretory products (ESPs) and tegument are critical for the pathogenesis, nutrient metabolism, etiology immune response of liver cancer. To understand functions various proteins in Clonorchis sinensis physiology human clonorchiasis, ESPs C. were identified. Supernatants containing from adult after culture 6 h harvested concentrated. The was detached using a freeze/thaw method successively extracted extraction buffers. outer surface labeled with biotin, biotinylated purified. ESP, identified analyzed by high-resolution LC-MS/MS. compared those other flukes, protein associated carcinogenesis potential vaccine antigens drug targets predicted analyzed. A total 175 6-h ESPs. 352 through sequential solubilization isolated teguments, subset these localized to membrane labeling biotin. Thirty annexins, actin tetraspanins, as immunomodulators promising antigens. Interestingly, among many 155 enzymes, most oxidoreductases, hydrolases or transferases. comparison flukes indicated that have some common such actin, tetraspanin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) annexin. Granulin, thioredoxin peroxiredoxin, carbonyl reductase 1 cystatin proteome be related disease analysis could contribute more in-depth understanding complex parasite-host relationships, improve diagnosis clonorchiasis benefit research on pathogenesis development novel interventions, drugs vaccines control infection.