HIV-1 protease inhibitors decrease proliferation and induce differentiation of human myelocytic leukemia cells.

摘要: Inhibitors of the protease human immunodeficiency virus type 1 (HIV-1) may inhibit cytoplasmic retinoic acid-binding proteins, cytochrome P450 isoforms, as well P-glycoproteins. These features inhibitors might enhance activity retinoids. To explore this hypothesis, myeloid leukemia cells were cultured with all- trans acid (ATRA) either alone or in combination HIV-1 indinavir, ritonavir, and saquinavir. Consistent enhanced ability ATRA to growth induce differentiation HL-60 NB4 cells, measured by expression CD11b CD66b cell surface antigens, reduction nitroblue tetrazolium. Growth ATRA-resistant UF-1 was also inhibited when indinavir. Moreover, indinavir differentiation-related transcription factor C/EBPe messenger RNA 9.5-fold. Taken together, results show that antiproliferative differentiating effects on cells. An inhibitor be a useful adjuvant for patients acute promyelocytic possibly retinoid-resistant cancers.