Quantification of fetomaternal hemorrhage: a comparative study of the manual and automated microscopic Kleihauer-Betke tests and flow cytometry in clinical samples.
作者： Denise M. Pelikan , Sicco A. Scherjon , Wilma E. Mesker , Godelieve M. de Groot-Swings , Geeske G. Brouwer-Mandema
DOI: 10.1016/J.AJOG.2004.01.007
关键词:
摘要: Abstract Objective The purpose of this study was to evaluate the quantification fetomaternal hemorrhage by manual and automated microscopic analysis Kleihauer-Betke stained slides flow cytometry. Study design Blood smears were evaluated manually according test. same used for microscopy. In addition, blood cytometry performed anti–hemaglobin F immunostaining. Results Fetomaternal >0.1% detected in 4 patients test absent all 3 methods 13 patients; Conclusion Automated detection fetal cells clinical samples provides accurate that is comparable both small large hemorrhage. capable only quantifying >0.1%.
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