The potential use of tomato lectin for oral drug delivery: 2. Mechanism of uptake in vitro

摘要: Abstract Tomato lectin (TL) is a non-toxic dietary glycoprotein of molecular mass 71 kDa. Its interaction with, and uptake by, the adult rat small intestine was investigated using an improved everted gut sac system, to evaluate its potential in oral drug delivery systems. Uptake [ 125 I]TL compared with two control molecules; I-labelled polyvinylpyrrolidone (PVP), inert polymer often used as marker for fluid-phase endocytosis, bovine serum albumin (BSA), degradable protein similar TL. substrate associated tissue or serosal space calculated ng per mg protein. The rate tomato into at 37°C 27 ng/h protein, but passage much slower (1.7 protein). by 11-times higher than BSA 20-times PVP, transfer only 4.3- 5-times greater respectively. This showed that proportion transferred across mucosa serosa less either control, indicated accumulation TL within enterocytes. Incubation 4°C metabolic inhibitors demonstrated mechanism intestinal sacs culture adsorptive endocytosis. Trichloroacetic acid solubility studies more resistant enzyme degradation during incubation BSA. fact shown adhere surface, increased when macromolecules, PVP BSA, accumulated mucosal cells, may give it carrier gastrointestinal tract.