Small interfering RNA targeting survivin sensitizes lung cancer cell with mutant p53 to adriamycin

摘要: Survivin is a member of the inhibitor apoptosis protein (IAP) family that specifically overexpressed in cancer tissues. p53 one tumor suppressor genes; its induction response to DNA damage causes and correlates with drug sensitivity. To investigate possible regulation survivin by p53, we examined level expression lung cell lines adriamycin. Levels mRNA wild-type decreased dramatically after induction, but no such reduction was observed mutated or null p53. Inhibition A549 cells small interfering (si) RNA significantly upregulated survivin. inhibition siRNA PC9 depressed proliferation. sensitivity adriamycin survivin, using siRNA, then added at an IC50 dose. After further 48 hr incubation adriamycin, proliferation treated targeting comparison scramble. Furthermore, both TUNEL pro-caspase3 assay showed significant increase combined treatment Our results demonstrate downregulated increases promotes apoptosis. could be potentially useful for increasing anticancer drugs, especially drug-resistant ' 2005 Wiley-Liss, Inc.