DCE-MRI using small-molecular and albumin-binding contrast agents in experimental carcinomas with different stromal content

摘要: OBJECTIVES: To compare DCE-MRI experiments performed using a standard small-molecular (Gd-DTPA) and an albumin-binding (MS-325) contrast agent in two carcinoma models with different stromal content. MATERIALS AND METHODS: DU-145 or BXPC-3 cancer cells were subcutaneously injected into nude mice. was by bolus injection of Gd-DTPA MS-325 about 2 weeks after inoculation. For quantitative analysis volume interest manually drawn over each tumor. address the heterogeneous enhancement, tumor then divided 20% most-enhancing remaining 80% least-enhancing fractions. Mean enhancement calculated these selected volumes compared between groups agents. Maps differential peak time-to-peak used for visual evaluation. CD31 VEGF immunohistochemistry excised tumors. RESULTS: In volume, at late time points dynamic scan, mean elicited higher than either agents, significant difference tumors types observed only early, while later scan obscured faster washout Enhancement maps confirmed that characterized marked rate agent, particularly enhancing peripheral rim. With this pattern appeared to be specific carcinomas, since it not Finally, both types, produced significantly phase scan. Ex vivo presence aberrant infiltrative stroma tumors, which vessels embedded. all central portion less viable infiltrated tissue areas. CONCLUSIONS: Contrast distribution proved related content, presumably particular, 'early' contrast-enhanced MRI, as most sensitive technique detect portions high i.e. rim Since same recently investigated FDG-PET imaging, showing inverse relationship FDG uptake MRI could provide complementary comprehensive sensitivity assessment carcinomas.