The effects of neonatal cryoanaesthesia-induced hypothermia on adult emotional behaviour and stress markers in C57BL/6 mice

摘要: Since the early 1930s, deep hypothermia (cryoanaesthesia) has been a useful anaesthetic in several types of surgery on neonatal rodents. Especially against background modern techniques systems neuroscience, method enjoys again increasing popularity. However, little is known about its effects subsequent adult behavioural and physiological profile. To systematically investigate cryoanaesthesia basal emotional behaviour as well development, 59 C57BL/6 mouse pups were randomly assigned to one three treatment groups: Pups first group exposed (H) postnatal day 3, while other two groups served controls: These either remained home cage without any intervention (C), or separated from mother for 15 min (MS) differentiate between isolation alone versus that inevitably goes along with isolation. Subsequent analyses conducted during adulthood (P 84-P 130), including tests exploratory, anxiety-like depression-like behaviour. At age 145 days mice decapitated record BDNF levels hippocampus serum corticosterone. Altogether, H found display slightly increased anxiety O-Maze, but did not differ control animals test. Subtle alterations behaviour, however, accompanied by changes corticosterone hippocampal levels, arguing an overall long-lasting effect profile mice.