Rates and product identification for trenbolone acetate metabolite biotransformation under aerobic conditions

摘要: Trenbolone acetate metabolites are endocrine-active contaminants discharged into the aquatic environment in runoff from agricultural fields, rangelands, and concentrated animal feeding operations. To investigate environmental fate of these compounds their biotransformation mechanisms, authors used inocula a variety different water sources dosed biologically active microcosms with approximately 1400 ng/L trenbolone metabolites, including 17β-trenbolone, trendione, 17α-trenbolone. aerobic rates interconversions between known gas chromatography-tandem mass spectrometry was to measure concentrations assess product distributions as function time. High-resolution liquid (LC-MS/MS) characterize novel transformation products potential pathways. Kinetic analysis yields observed half-lives 0.9 d, 1.3 2.2 d for 17α-trenbolone, respectively, at 20 °C, although colder conditions increased 8.5 biphasic observed. Relative reported faster attenuation soils, likely more persistent aqueous systems. Product indicate an enzymatic preference trendione 17β-trenbolone. The LC-MS/MS characterization indicates dehydrogenation major detectable demonstrates that structural elements responsible bioactivity steroids retained during biotransformation.