Fast preparation of hybrid monolithic columns via photo-initiated thiol-yne polymerization for capillary liquid chromatography.

摘要: Abstract Although several approaches have been developed to fabricate hybrid monoliths, it would still take a few hours finish the formation of monoliths. Herein, photo-initiated thiol-yne polymerization was first adopted in situ monoliths within confines UV-transparent fused-silica capillary. A silicon-containing diyne (1,3-diethynyltetramethyl-disiloxane, DYDS) copolymerized with three multithiols, 1,6-hexanedithiol, trimethylolpropane tris(3-mercaptopropionate) and pentaerythriol tetrakis(3-mercaptopropionate), by using binary porogenic system diethylene glycol diethyl ether (DEGDE)/poly(ethylene glycol) (PEG200) 10 min. Several characterizations (assigned as I, II III, respectively) were performed. The results showed that these possessed bicontinuous porous structure, which remarkably different from via typical free-radical polymerization. highest column efficiency 76,000 plates per meter for butylbenzene obtained on I reversed-phase liquid chromatography (RPLC). It observed efficiencies strong-retained almost close those weak-retained benzene, indicating retention-independent efficient performance small molecules I. surface area this monolith very dry state (less than 10.0 m2/g), chromatographic behavior monolithic columns be possibly explained radical-mediated step-growth process Finally, applied separation BSA tryptic digest cLC–MS/MS, satisfactory ability complicated samples.