Effect of Low-Dose Aspirin on Candidate Biomarkers in the Urine Proteome

摘要: A biomarker is a measurable change associated with physiological or pathophysiological process. Unlike blood, which homeostatic, urine more likely to reflect changes in the body; also promising source of discovery because physiology are accumulated this fluid. However, urinary remains major challenge proteome influenced by various factors. Low-dose aspirin widely used for preventing myocardial infarction, ischemic stroke and vascular death among patients at both high low risk these events as well some cancers. Because animal models allow controlling genetic environmental factors, study, effects on were analysed rats. Urinary samples collected before 7 days after administration low-dose (50mg/ kg.d). total 191 proteins identified Nano LC-MS/MS then quantified using Scaffold software. Twelve significantly altered, suggesting that affects proteome. Nine 12 have been previously reported candidate disease biomarkers, must be considered future research. Additionally, protein induced distinct from those caused heparin argatroban treatments; indicates can distinguish blood coagulation status different mechanisms, may help design specific treatment plans patients. Two up-regulated proteins, pro-EGF CD10, selected further validation western blotting, results consistent mass spectrometry. Therefore, we propose two monitoring platelet aggregation.