Complementary Critical Functions of Zfy1 and Zfy2 in Mouse Spermatogenesis and Reproduction.

摘要: The mammalian Y chromosome plays a critical role in spermatogenesis. However, the exact functions of each gene have not been completely elucidated, partly owing to difficulties targeting analysis chromosome. Zfy was first proposed be sex determination factor, but its function spermatogenesis has recently elucidated. Nevertheless, performed thus far. Here, we adopted highly efficient CRISPR/Cas9 system generate individual Zfy1 or Zfy2 knockout (KO) mice and double (Zfy1/2-DKO) mice. While Zfy2-KO did show any significant phenotypic alterations fertility, Zfy1/2-DKO were infertile displayed abnormal sperm morphology, fertilization failure, early embryonic development failure. Mass spectrometric screening, followed by confirmation with western blot analysis, showed that PLCZ1, PLCD4, PRSS21, HTT protein expression significantly deceased spermatozoa compared those wild-type These results are consistent changes seen double-mutant Collectively, our strategy findings revealed redundant spermatogenesis, facilitating better understanding failure