Elimination of Primer-Dimer Artifacts and Genomic Coamplification Using a Two-Step SYBR Green I Real-Time RT-PCR

Russell Higuchi, Carita Fockler, Gavin Dollinger, Robert Watson, Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions Nature Biotechnology. ,vol. 11, pp. 1026- 1030 ,(1993) , 10.1038/NBT0993-1026

Yolanda S Lie, Christos J Petropoulos, Advances in quantitative PCR technology: 5' nuclease assays. Current Opinion in Biotechnology. ,vol. 9, pp. 43- 48 ,(1998) , 10.1016/S0958-1669(98)80082-7

Jannine Brownie, Susan Shawcross, Jane Theaker, David Whitcombe, Richard Ferrie, Clive Newton, Stephen Little, The elimination of primer-dimer accumulation in PCR. Nucleic Acids Research. ,vol. 25, pp. 3235- 3241 ,(1997) , 10.1093/NAR/25.16.3235

SA Bustin, Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. Journal of Molecular Endocrinology. ,vol. 25, pp. 169- 193 ,(2000) , 10.1677/JME.0.0250169

Kirk M. Ririe, Randy P. Rasmussen, Carl T. Wittwer, Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction Analytical Biochemistry. ,vol. 245, pp. 154- 160 ,(1997) , 10.1006/ABIO.1996.9916

C A Heid, J Stevens, K J Livak, P M Williams, Real time quantitative PCR. Genome Research. ,vol. 6, pp. 986- 994 ,(1996) , 10.1101/GR.6.10.986

Masahira Hattori, A Fujiyama, TD Taylor, H Watanabe, T Yada, H-S Park, A Toyoda, K Ishii, Y Totoki, D-K Choi, E Soeda, M Ohki, T Takagi, Y Sakaki, S Taudien, K Blechschmidt, A Polley, U Menzel, J Delabar, K Kumpf, R Lehmann, D Patterson, K Reichwald, A Rump, M Schillhabel, A Schudy, W Zimmermann, A Rosenthal, J Kudoh, K Shibuya, K Kawasaki, S Asakawa, A Shintani, T Sasaki, K Nagamine, S Mitsuyama, SE Antonarakis, S Minoshima, N Shimizu, G Nordsiek, K Hornischer, P Brandt, M Scharfe, O Schön, A Desario, J Reichelt, G Kauer, H Blöcker, J Ramser, A Beck, S Klages, S Hennig, L Riesselmann, E Dagand, T Haaf, S Wehrmeyer, K Borzym, K Gardiner, D Nizetic, F Francis, H Lehrach, R Reinhardt, M-L Yaspo, The DNA sequence of human chromosome 21 Nature. ,vol. 405, pp. 311- 319 ,(2000) , 10.1038/35012518

I Dunham, AR Hunt, JE Collins, R Bruskiewich, DM Beare, M Clamp, LJ Smink, R Ainscough, JP Almeida, A Babbage, C Bagguley, J Bailey, K Barlow, KN Bates, O Beasley, CP Bird, S Blakey, AM Bridgeman, D Buck, J Burgess, WD Burrill, J Burton, C Carder, NP Carter, Y Chen, G Clark, SM Clegg, V Cobley, CG Cole, RE Collier, RE Connor, D Conroy, N Corby, GJ Coville, AV Cox, J Davis, E Dawson, PD Dhami, C Dockree, SJ Dodsworth, RM Durbin, A Ellington, KL Evans, JM Fey, K Fleming, L French, AA Garner, JGR Gilbert, ME Goward, D Grafham, MN Griffiths, C Hall, R Hall, G Hall-Tamlyn, RW Heathcott, S Ho, S Holmes, SE Hunt, MC Jones, J Kershaw, A Kimberley, A King, GK Laird, CF Langford, MA Leversha, C Lloyd, DM Lloyd, ID Martyn, M Mashreghi-Mohammadi, L Matthews, OT McCann, J McClay, S McLaren, AA McMurray, SA Milne, BJ Mortimore, CN Odell, R Pavitt, AV Pearce, D Pearson, BJ Phillimore, SH Phillips, RW Plumb, H Ramsay, Y Ramsey, L Rogers, MT Ross, CE Scott, HK Sehra, CD Skuce, S Smalley, ML Smith, C Soderlund, L Spragon, CA Steward, JE Sulston, RM Swann, M Vaudin, M Wall, JM Wallis, MN Whiteley, D Willey, L Williams, S Williams, H Williamson, TE Wilmer, L Wilming, CL Wright, T Hubbard, DR Bentley, S Beck, J Rogers, N Shimizu, S Minoshima, K Kawasaki, T Sasaki, S Asakawa, J Kudoh, A Shintani, K Shibuya, Y Yoshizaki, N Aoki, S Mitsuyama, BA Roe, F Chen, L Chu, J Crabtree, S Deschamps, A Do, T Do, A Dorman, F Fang, Y Fu, P Hu, A Hua, S Kenton, H Lai, HI Lao, J Lewis, S Lewis, S-P Lin, P Loh, E Malaj, Thanh Nguyen, H Pan, S Phan, S Qi, Y Qian, L Ray, Q Ren, None, The DNA sequence of human chromosome 22 Nature. ,vol. 402, pp. 489- 495 ,(1999) , 10.1038/990031