In vitro and in vivo experiments with iron oxide nanoparticles functionalized with DEXTRAN or polyethylene glycol for medical applications: magnetic targeting.

摘要: In this research work, DEXTRAN- and polyethyl- ene glycol (PEG)-coated iron-oxide superparamagnetic nano- particles were synthetized their cytotoxicity biodistribution assessed. Well-crystalline hydrophobic Fe3O4 SPIONs formed by a thermal decomposition process with d 5 18 nm r 2 nm; finally, the character of was changed to hydrophilic post-synthesis pro- cedure functionalization PEG or DEXTRAN. The nanoparticles present high saturation mag- netization behavior at room tempera- ture, hydrodynamic diameters PEG-coated measured as 170 120 nm, respectively. PEG- DEXTRAN-coated have Spe- cific Power Absorption SPA 320 400 W/g, respectively, in an ac magnetic field amplitude 13 kA/m fre- quency 256 kHz. vitro studies using VERO MDCK cell lineages performed study uptake SPIONs. For both lineages, presented viability for concentrations 200 lg/mL. vivo conducted BALB/c mice inoculating intrave- nously exposing them presence external magnet located over tumour. It observed that amount tumor increased up 160% when permanent opposed those animals not exposed exter- nal field. V C