Human colorectal adenocarcinoma cells: differential nitric oxide synthesis determines their ability to aggregate platelets.

摘要: Abstract The existence and role of an l-arginine:nitric oxide (NO) pathway in two human colorectal adenocarcinoma cell lines, SW-480 SW-620, were investigated. Both which derive from the same patient, primary tumor SW-620 its metastatic lesion, shown to have a cytosolic, Ca2+-independent, NADPH-dependent NO synthase, activity was lower cytosol SW-620. These cells more potent inducers platelet aggregation. In contrast, SW-480, had synthase activity, less Pretreatment both lines with NG-monomethyl-l-arginine, inhibitor potentiated their proaggregating effect made them equally active. Exogenous l-arginine, NO, related nitrovasodilators all inhibited aggregation induced by antiaggregating further prostacyclin M&B22948, selective cyclic GMP phosphodiesterase. We propose that generation inversely correlates potential. Furthermore, we show is due presence these low molecular weight synthase. addition, agents modulate function GMP-dependent mechanism may be useful prevention metastasis.