High-performance liquid chromatography (HPLC) assay for ribavirin and comparison of the HPLC assay with radioimmunoassay.

摘要: The use of high-performance liquid chromatography (HPLC) to measure ribavirin in serum and other biological fluids has been limited by endogenous interfering substances. We report an HPLC procedure based on the extraction from serum, plasma, or cerebrospinal fluid with a boronate affinity gel, which uses 3-methylcytidine internal standard. This assay is sensitive (to 0.4 microM), specific (no interference 34 commonly prescribed drugs), reproducible (coefficients variation 5.4 22.4%), linear (r = 0.999) over range clinically relevant concentrations (from 0.5 50.0 microM). It also correlates well radioimmunoassay 0.992). should be useful for measuring body during clinical trials.