Molecular Portraits of Epithelial, Mesenchymal, and Hybrid States in Lung Adenocarcinoma and Their Relevance to Survival

摘要: Epithelial to mesenchymal transition (EMT) is a key process associated with tumor progression and metastasis. To define molecular features EMT states, we undertook an integrative approach combining mRNA, microRNA, DNA methylation proteomic profiles of 38 cell populations representative the genomic heterogeneity in lung adenocarcinoma were integrated functional consisting invasiveness, adhesion motility. A subset lines that readily defined as epithelial or based on their morphology E-cadherin vimentin expression elicited distinctive signatures. However, most displayed intermediate/hybrid states EMT, mixed characteristics. dominant feature aggressive hybrid was upregulation cytoskeletal actin binding proteins, signature shared lines. Cytoskeletal reorganization preceded loss cells which induced by TGFβ. set transcripts corresponding protein enriched proteins found be predictive survival independent datasets adenocarcinomas. Our findings point association between actin-binding phenotype invasive properties