Effects of encapsulated rabbit mesenchymal stem cells on ex vivo expansion of human umbilical cord blood hematopoietic stem/progenitor cells.
作者： Yang Liu , Tianqing Liu , Xuehu Ma , Xiubo Fan , Chunyu Bao
DOI: 10.1080/02652040802193014
关键词:
摘要: The expansion of umbilical cord blood mononuclear cells (UCB MNCs) was investigated in a novel co-culture system by means encapsulation rabbit bone marrow (BM) mesenchymal stem (MSCs) alginate beads (Alg beads). Three kinds media were applied and the experiments lasted for 7 days. total nucleated cell density measured every 24 h. Flow cytometric assay CD34+ methylcellulose colony assays carried out at 0, 72 168 It found that encapsulated MSCs illustrated remarkable effects on UCB MNCs regardless whether serum is present culture or not. At end h co-culture, number multiplied 15 ± 2.9 times, 5.3 0.3 times colony-forming units (CFU-Cs) 5.6 1.2 serum-free supplemented with conventional dose cytokines, which very similar to results containing 20% media. While control, i.e. MNC without encapsula...
informapharmascience.com参考文章(45)
Thomas Noll, Nanni Jelinek, Sebastian Schmidt, Manfred Biselli, Christian Wandrey, Cultivation of Hematopoietic Stem and Progenitor Cells: Biochemical Engineering Aspects Advances in Biochemical Engineering \/ Biotechnology. ,vol. 74, pp. 111- 128 ,(2002) , 10.1007/3-540-45736-4_6
Mutsumi Takagi, Ken Horii, Toshiomi Yoshida, Effect of pore diameter of a porous membrane on progenitor cell content during membrane-separated coculture of hematopoietic cells and a stromal cell line Journal of Artificial Organs. ,vol. 6, pp. 130- 137 ,(2003) , 10.1007/S10047-003-0220-1
Brian Johnstone, Thomas M. Hering, Arnold I. Caplan, Victor M. Goldberg, Jung U. Yoo, In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells Experimental Cell Research. ,vol. 238, pp. 265- 272 ,(1998) , 10.1006/EXCR.1997.3858
Jon A. Rowley, Gerard Madlambayan, David J. Mooney, Alginate hydrogels as synthetic extracellular matrix materials Biomaterials. ,vol. 20, pp. 45- 53 ,(1999) , 10.1016/S0142-9612(98)00107-0
M A Slatter, A R Gennery, Umbilical cord stem cell transplantation for primary immunodeficiencies. Expert Opinion on Biological Therapy. ,vol. 6, pp. 555- 565 ,(2006) , 10.1517/14712598.6.6.555
John P. Chute, Garrett G. Muramoto, Jennifer Fung, Carol Oxford, Soluble factors elaborated by human brain endothelial cells induce the concomitant expansion of purified human BM CD34+CD38- cells and SCID-repopulating cells. Blood. ,vol. 105, pp. 576- 583 ,(2005) , 10.1182/BLOOD-2004-04-1467
Jonathon F. Hutton, Vladislav Rozenkov, Fiona S.L. Khor, Richard J. D'Andrea, Ian D. Lewis, Bone Morphogenetic Protein 4 Contributes to the Maintenance of Primitive Cord Blood Hematopoietic Progenitors in an Ex Vivo Stroma-Noncontact Co-Culture System Stem Cells and Development. ,vol. 15, pp. 805- 813 ,(2006) , 10.1089/SCD.2006.15.805
Steven Masson, David J Harrison, John N Plevris, Philip N Newsome, Potential of Hematopoietic Stem Cell Therapy in Hepatology: A Critical Review Stem Cells. ,vol. 22, pp. 897- 907 ,(2004) , 10.1634/STEMCELLS.22-6-897
I. McNiece, J. Harrington, J. Turney, J. Kellner, E.J. Shpall, Ex vivo expansion of cord blood mononuclear cells on mesenchymal stem cells Cytotherapy. ,vol. 6, pp. 311- 317 ,(2004) , 10.1080/14653240410004871
Xiangqin Li, Tianqing Liu, Kedong Song, Lisong Yao, Dan Ge, Chunyu Bao, Xuehu Ma, Zhanfeng Cui, None, Culture of neural stem cells in calcium alginate beads. Biotechnology Progress. ,vol. 22, pp. 1683- 1689 ,(2006) , 10.1021/BP060185Z