Encapsulated feeder cells within alginate beads for ex vivo expansion of cord blood-derived CD34+ cells
作者： Xiuwei Pan , Qiong Sun , Haibo Cai , Yun Gao , Wensong Tan
DOI: 10.1039/C6BM00191B
关键词:
摘要: A co-culture system based on encapsulated feeder cells within alginate beads was developed through optimizing the detailed aspects of cell culture to expand CD34-positive (CD34+) ex vivo. Mesenchymal stem isolated from different sources (human amniotic (hAMSCs) and umbilical cord (UCMSCs)), human fibroblast (HFs) have been respectively chosen as results showed that hAMSCs were superior UCMSCs HFs in conventional two-dimensional (2D) co-cultures regarding promotion total nucleated (TNCs) expansion maintenance CD34+ phenotype. Alginate employed limit growth hAMSCs, which could effectively restrict proliferation while viability still highly maintained. Intriguingly, only a few migrated out beads, whereas secreted factors cytokines be easily released. Furthermore, supported cells/hAMSCs 2D indirect exhibited increased TNCs expansion, higher percentages CD34+CD38− cells, better vitality when compared co-culture. Therefore, can promote
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sci-hub.se 参考文章(61)
Maria Ester Bernardo, Willem E. Fibbe, Mesenchymal stromal cells and hematopoietic stem cell transplantation. Immunology Letters. ,vol. 168, pp. 215- 221 ,(2015) , 10.1016/J.IMLET.2015.06.013
Bhushan P. Mahadik, Sara Pedron Haba, Luke J. Skertich, Brendan A.C. Harley, The use of covalently immobilized stem cell factor to selectively affect hematopoietic stem cell activity within a gelatin hydrogel Biomaterials. ,vol. 67, pp. 297- 307 ,(2015) , 10.1016/J.BIOMATERIALS.2015.07.042
RS Taichman, MJ Reilly, SG Emerson, Human osteoblasts support human hematopoietic progenitor cells in vitro bone marrow cultures Blood. ,vol. 87, pp. 518- 524 ,(1996) , 10.1182/BLOOD.V87.2.518.BLOODJOURNAL872518
Raymond Schofield, The relationship between the spleen colony-forming cell and the haemopoietic stem cell Blood cells. ,vol. 4, pp. 7- 25 ,(1978)
X JIANG, C CHAI, Y ZHANG, R ZHUO, H MAO, K LEONG, Surface-immobilization of adhesion peptides on substrate for ex vivo expansion of cryopreserved umbilical cord blood CD34+ cells. Biomaterials. ,vol. 27, pp. 2723- 2732 ,(2006) , 10.1016/J.BIOMATERIALS.2005.12.001
H. E. Broxmeyer, G. W. Douglas, G. Hangoc, S. Cooper, J. Bard, D. English, M. Arny, L. Thomas, E. A. Boyse, Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 3828- 3832 ,(1989) , 10.1073/PNAS.86.10.3828
William L. Murphy, Todd C. McDevitt, Adam J. Engler, Materials as stem cell regulators Nature Materials. ,vol. 13, pp. 547- 557 ,(2014) , 10.1038/NMAT3937
Simón Méndez-Ferrer, Tatyana V. Michurina, Francesca Ferraro, Amin R. Mazloom, Ben D. MacArthur, Sergio A. Lira, David T. Scadden, Avi Ma’ayan, Grigori N. Enikolopov, Paul S. Frenette, Mesenchymal and haematopoietic stem cells form a unique bone marrow niche Nature. ,vol. 466, pp. 829- 834 ,(2010) , 10.1038/NATURE09262
Ruijiao Dong, Yan Pang, Yue Su, Xinyuan Zhu, Supramolecular hydrogels: synthesis, properties and their biomedical applications Biomaterials Science. ,vol. 3, pp. 937- 954 ,(2015) , 10.1039/C4BM00448E
Jon A. Rowley, Gerard Madlambayan, David J. Mooney, Alginate hydrogels as synthetic extracellular matrix materials Biomaterials. ,vol. 20, pp. 45- 53 ,(1999) , 10.1016/S0142-9612(98)00107-0