Efficient cryopreservation of humpback grouper, Cromileptes altivelis (Valenciennes, 1828) spermatozoa.

作者: Renu Yahsiro , Suriyan Tunkijjanukij , Nipon Sean-in , Samorn Ponchunchoovong

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摘要: Chilled storage of humpback grouper, Cromileptes altivelis (Valenciennes, 1828) sperm cells with five extenders was investigated to find the most suitable type extender for cryopreservation. Five extenders, namely Marine Fish Ringer (MFR), Extender 251 (E 251), 189 189), 0.1 M Sodium Citrate (CT) and 0.9% NaCl (NaCl) were tested. They proved be appropriate use since no motile all still alive at 216 h (60 after chilled storage). There also significant difference (p 0.05) between fresh sperms cryopreserved in 5% DMSO, but had a (p<0.05) those DMA.]

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