EC300: a phage-based, bacteriolysin-like protein with enhanced antibacterial activity against Enterococcus faecalis

摘要: Bacteriophage lytic enzymes, either endolysins or virion-associated lysins, have been receiving considerable attention as potential antibacterial agents, particularly for the combat of antibiotic-resistant Gram-positive pathogens. A conclusion that easily emerges from careful analysis a great number reports on field is activity phage enzymes rarely studied in conditions support robust growth target bacteria. Here, we report construction and study chimerical lysin, EC300, which was designed to kill Enterococcus faecalis supporting vigorous bacterial growth. EC300 resulted fusion predicted M23 endopeptidase domain lysin putative cell wall binding previously characterized amidase endolysin, both produced by E. F170/08. This bacteriolysin-like protein exhibited clear enhanced over parental endolysin when were assayed rich medium. We demonstrate killing efficacy against growing cells panel typed clinical strains with high level antibiotic resistance. The possible reasons marked difference between performance are discussed.