Overlapping epitopes that are recognized by CD8+ HLA class I-restricted and CD4+ class II-restricted cytotoxic T lymphocytes are contained within an influenza nucleoprotein peptide.

摘要: Viral epitopes that are recognized by both HLA class I-restricted and II-restricted T cells have been defined for a type A influenza virus nucleoprotein (NP) peptide. CD8+ CD4+ CTL lines generated against synthetic peptide encompassing residues 335 to 349 of NP restricted HLA-B37 HLA-DQw5, respectively. Both these populations were capable specifically lysing virus-infected targets, indicating naturally processed peptide(s) was being mimicked the (335-349) Amino acid critical recognition this determinant in context HLA-DQw5 investigated use panels truncated alanine-substituted peptides. The results demonstrate that: 1) truncations amino- or carboxy-terminal ends differentially affect recognition; 2) sequence contains two octapeptide share core six amino (NP 338-343); 3) alanine substitutions at five abrogated least one lines. Thus, I- recognize similar but distinct epitopes, different structural features required presentation HLA-DQw5. Comparison sequences presented with other peptides known be I II molecules revealed common motif among