Expression and characterization of biologically active ovine FSH from mammalian cell lines

摘要: Stably transfected cell lines expressing the alpha subunit, beta subunit and alpha/beta heterodimer of ovine (o)FSH have been established following transfection Chinese hamster ovary cells with cDNA expression vectors. In absence FSH polypeptides were inefficiently secreted displayed a short intracellular half-life, while free subunits readily in subunit. Cotransfection oFSH cDNAs led to assembly secretion. While alteration nucleotide sequence flanking AUG initiation codon did not appreciably enhance biosynthesis secretion, replacement 5' untranslated signal peptide-coding regions corresponding sequences from an oGH clone was associated twofold increase The recombinant had higher molecular weight than pituitary-derived oFSH, more acidic native hormone when analysed using isoelectric focusing, suggesting greater degree sialylation hormone. A comparison activities hormones porcine testis radioreceptor assay vitro Sertoli bioassay revealed that enhanced biological activity compared