Co-expression of human protein disulfide isomerase (hPDI) enhances secretion of bovine follicle-stimulating hormone (bFSH) in Pichia pastoris.
作者： Xiangdong Huo , Yueyong Liu , Xu Wang , Pingkai Ouyang , Zhengdong Niu
DOI: 10.1016/J.PEP.2007.03.016
关键词:
摘要: Bovine follicle-stimulating hormone (bFSH) is a pituitary gonadotropin composed of two non-covalently associated polypeptide subunits, which must be glycosylated, folded, and assembled as heterodimer to biologically active. Low-level expression the recombinant bFSH factor that limits its usefulness superovulation treatment for cows. To increase production bFSH, human protein disulfide isomerase (hPDI) was expressed simultaneously in engineered Pichia strains. The secretion characteristics with or without hPDI were examined. co-expression increased 1.56 mg/l culture medium, 6-fold higher when compared control strain carrying gene only. These results may generally applicable other glycoprotein hormones yeast.
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sci-hub.se 参考文章(38)
Shixuan Wu, Geoffrey J. Letchworth, High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol. BioTechniques. ,vol. 36, pp. 152- 154 ,(2004) , 10.2144/04361DD02
J L Keene, M M Matzuk, T Otani, B C Fauser, A B Galway, A J Hsueh, I Boime, Expression of biologically active human follitropin in Chinese hamster ovary cells. Journal of Biological Chemistry. ,vol. 264, pp. 4769- 4775 ,(1989) , 10.1016/S0021-9258(18)83656-0
Dietmar Dirnberger, Herta Steinkellner, Latifa Abdennebi, Jean-Jacques Remy, Dick van de Wiel, Secretion of biologically active glycoforms of bovine follicle stimulating hormone in plants. FEBS Journal. ,vol. 268, pp. 4570- 4579 ,(2001) , 10.1046/J.1432-1327.2001.02384.X
N. M. Greenberg, J. W. Anderson, A. J. Hsueh, K. Nishimori, J. J. Reeves, D. M. deAvila, D. N. Ward, J. M. Rosen, Expression of biologically active heterodimeric bovine follicle-stimulating hormone in milk of transgenic mice. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 8327- 8331 ,(1991) , 10.1073/PNAS.88.19.8327
Hubertus Hohenblum, Brigitte Gasser, Michael Maurer, Nicole Borth, Diethard Mattanovich, Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris. Biotechnology and Bioengineering. ,vol. 85, pp. 367- 375 ,(2004) , 10.1002/BIT.10904
Protein disulfide isomerase mutant lacking its isomerase activity accelerates protein folding in the cell FEBS Letters. ,vol. 377, pp. 505- 511 ,(1995) , 10.1016/0014-5793(95)01410-1
Anne Skaja Robinson, Victoria Hines, K. Dane Wittrup, Protein Disulfide Isomerase Overexpression Increases Secretion of Foreign Proteins in Saccharomyces cerevisiae Nature Biotechnology. ,vol. 12, pp. 381- 384 ,(1994) , 10.1038/NBT0494-381
Fabien Richard, Philippe Robert, Jean-Jacques Remy, Nadine Martinat, Jean-Michel Bidart, Roland Salesse, Yves Combarnous, High-Level Secretion of Biologically Active Recombinant Porcine Follicle-Stimulating Hormone by the Methylotrophic YeastPichia pastoris Biochemical and Biophysical Research Communications. ,vol. 245, pp. 847- 852 ,(1998) , 10.1006/BBRC.1998.8532
P S Mountford, M R Brandon, T E Adams, Expression and characterization of biologically active ovine FSH from mammalian cell lines Journal of Molecular Endocrinology. ,vol. 12, pp. 71- 83 ,(1994) , 10.1677/JME.0.0120071
SANFORD L. STEELMAN, FLORENCE M. POHLEY, ASSAY OF THE FOLLICLE STIMULATING HORMONE BASED ON THE AUGMENTATION WITH HUMAN CHORIONIC GONADOTROPIN Endocrinology. ,vol. 53, pp. 604- 616 ,(1953) , 10.1210/ENDO-53-6-604