Association of nerve growth factor receptors with the triton X-100 cytoskeleton of PC12 cells

摘要: Triton X-100 solubilizes membranes of PC12 cells and leaves behind a nucleus an array cytoskeletal filaments. Nerve growth factor (NGF) receptors (10% those found in intact cells) are associated with this X-100-insoluble residue. Two classes NGF on which display rapid slow dissociating kinetics. Although rapidly binding is predominant (greater than 75%) cells, the majority to cytoskeleton slowly 75%). Rapidly can be converted form by plant lectin wheat germ agglutinin (WGA). This also increases number associate more 10-fold. 125I-NGF bound visualized light microscopy autoradiography residues cell bodies, as well cones neurites. The WGA-induced association cytoskeleton, however, not specific for receptor, since greater 90% surface glycoprotein WGA become material at concentrations 33 micrograms/ml. Concentrations change solubility membrane glycoproteins similar required alter kinetic state receptor. Both events may related crossbridging proteins induced multivalent lectin.