Methotrexate effects on folate status and the deoxyuridine suppression test in rats

摘要: Abstract Methotrexate (MTX) was administered to rats produce marked folate deficiency develop an experimental model for studying coenzyme abnormalities. Male Sprague-Dawley were fed control and experimental, folate-deficient diets, with or without MTX (0.1 mg/100 g body wt) i.p. every other day 2 wk. The MTX-treated developed severe signs, including diarrhea impaired growth. Cytological features of megaloblastic anemia seen in peripheral blood the group (FD)+MTX RBC macroovalocytosis, anisocytosis elevated neutrophil lobe counts, comparison both MTX-untreated FD rats. Urinary formiminoglutamic acid (FIGLU) excretion depressed these FD+MTX dU suppression test isolated lymphocyte cultures showed abnormal higher [ 3 H]thymidine uptake by DNA untreated groups at 11 wk, but only FD-MTX 5 wk; this associated lower plasma values. Thus, results indicate that have a more than produced diet alone.