ASSAY FOR FOLIC ACID ACTIVITY IN BLOOD
作者： Jack M. Cooperman
DOI: 10.1016/B978-0-12-403502-7.50044-2
关键词: Saline 、 Microorganism 、 Biology 、 Skimmed milk 、 Agar 、 Folic acid 、 Lactobacillus casei 、 Bacteria 、 Food science 、 Biochemistry 、 Streptococcus
摘要: Publisher Summary The three bacteria usually used for folic acid activity assays are Lactobacillus casei, Streptococcus faecalis (ATCC 8043), and Pediococcus cerevisiae 8081). These microorganisms differ in their ability to utilize naturally occurring folates. riboflavin assay medium needs be rehydrated according label directions. skim milk extract additional water is added so that the final volume twice given on of medium. solution then a single strength When an set up utilizing either one bacteria, daily agar stab transferred into broth previous day allowed incubate from 16 20 h at 37°. resulting thick bacterial suspension washed several times with sterile 0.9% saline minimize carryover folates
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