Stimulated Emission Depletion Microscopy
作者： Hans Blom , Jerker Widengren
DOI: 10.1021/ACS.CHEMREV.6B00653
关键词:
摘要: Despite its short history, diffraction-unlimited fluorescence microscopy techniques have already made a substantial imprint in the biological sciences. In this review, we describe how stimulated emission depletion (STED) imaging originally evolved, it compares to other optical super-resolution techniques, and what advantages provides compared previous golden-standards for microscopy, such as diffraction-limited electron microscopy. We outline prerequisites successful STED experiments, emphasizing equally critical roles of instrumentation, sample preparation, photophysics, major evolving strategies push borders even further life science. Finally, provide examples nanoscopy can be applied, within three different fields with particular potential experiments: neuroscience, plasma membrane biophysics, subcellular clinical diagnostics. these areas, and...
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