Photobleaching of YOYO-1 in Super-Resolution Single DNA Fluorescence Imaging

摘要: Super-resolution imaging of single DNA molecules via point accumulation for in nanoscale topography (PAINT) has great potential to visualize fine structures with nanometer resolution. In a typical PAINT video acquisition, dye (YOYO-1) solution sparsely bind the target surfaces (DNA) whose locations can be mathematically determined by fitting their fluorescent spread function. Many YOYO-1 intercalate into and remain there during imaging, most them have temporarily or permanently fluorescently bleached, often stochastically, allow visualization few events per frame video. Thus, controlling fluorescence on–off rate is important PAINT. this paper, we study photobleaching its correlation quality images. At low excitation laser power density, too slow minimum required density was identified, which theoretically predicted proposed method report.