Three-dimensional super-resolution fluorescence imaging of DNA
作者： Sevim Yardimci , Daniel R. Burnham , Samantha Y. A. Terry , Hasan Yardimci
DOI: 10.1101/796151
关键词:
摘要: Abstract Recent advances in fluorescence super-resolution microscopy are providing important insights into details of cellular structures. To acquire three dimensional (3D) images DNA, we combined binding activated localization (BALM) using fluorescent double-stranded DNA intercalators and optical astigmatism. We quantitatively establish the advantage mono-over bis-intercalators before demonstrating approach by visualizing single molecules stretched between microspheres at various heights. Finally, is applied to more complex environment intact damaged metaphase chromosomes, unravelling their structural features.
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