Three-dimensional super-resolution fluorescence imaging of DNA.
作者： Sevim Yardimci , Daniel R. Burnham , Samantha Y. A. Terry , Hasan Yardimci
DOI: 10.1038/S41598-020-68892-5
关键词:
摘要: Recent advances in fluorescence super-resolution microscopy are providing important insights into details of cellular structures. To acquire three dimensional (3D) images DNA, we combined binding activated localization (BALM) using fluorescent double-stranded DNA intercalators and optical astigmatism. We quantitatively establish the advantage bis- over mono-intercalators before demonstrating the approach by visualizing single DNA molecules stretched between microspheres at various heights. Finally, approach is applied to more complex environment intact damaged metaphase chromosomes, unravelling their structural features.
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J. E. House, Principles of chemical kinetics Academic Press. ,(2007)
Ralf Jungmann, Maier S Avendaño, Johannes B Woehrstein, Mingjie Dai, William M Shih, Peng Yin, Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT Nature Methods. ,vol. 11, pp. 313- 318 ,(2014) , 10.1038/NMETH.2835
Purushottam Babu Tiwari, Xuewen Wang, Jin He, Yesim Darici, Analyzing surface plasmon resonance data: choosing a correct biphasic model for interpretation. Review of Scientific Instruments. ,vol. 86, pp. 035001- ,(2015) , 10.1063/1.4914027
Joan-Ramon Daban, Electron microscopy and atomic force microscopy studies of chromatin and metaphase chromosome structure. Micron. ,vol. 42, pp. 733- 750 ,(2011) , 10.1016/J.MICRON.2011.05.002
Tatsuo Ushiki, Osamu Hoshi, Atomic force microscopy for imaging human metaphase chromosomes. Chromosome Research. ,vol. 16, pp. 383- 396 ,(2008) , 10.1007/S10577-008-1241-7
Daniel J. Metcalf, Rebecca Edwards, Neelam Kumarswami, Alex E. Knight, Test Samples for Optimizing STORM Super-Resolution Microscopy Journal of Visualized Experiments. pp. 50579- ,(2013) , 10.3791/50579
Jonas Ries, Enrico Klotzsch, Helge Ewers, Viola Vogel, Ingmar Schoen, Binding-Activated Localization Microscopy of DNA Structures Nano Letters. ,vol. 11, pp. 4008- 4011 ,(2011) , 10.1021/NL2025954
Hasan Yardimci, Xindan Wang, Anna B. Loveland, Inger Tappin, David Z. Rudner, Jerard Hurwitz, Antoine M. van Oijen, Johannes C. Walter, Bypass of a protein barrier by a replicative DNA helicase Nature. ,vol. 492, pp. 205- 209 ,(2012) , 10.1038/NATURE11730
Jeffrey M Levsky, Robert H Singer, Fluorescence in situ hybridization: past, present and future. Journal of Cell Science. ,vol. 116, pp. 2833- 2838 ,(2003) , 10.1242/JCS.00633
A. Sfeir, T. de Lange, Removal of Shelterin Reveals the Telomere End-Protection Problem Science. ,vol. 336, pp. 593- 597 ,(2012) , 10.1126/SCIENCE.1218498