Bulk and single-molecule fluorescence studies of the saturation of the DNA double helix using YOYO-3 intercalator dye.
作者： Sergio G. Lopez , Maria J. Ruedas-Rama , Salvador Casares , Jose M. Alvarez-Pez , Angel Orte
DOI: 10.1021/JP303438D
关键词:
摘要: We report a thorough photophysical characterization of the interactions between double-stranded DNA (dsDNA) and trimethine cyanine homodimer dye YOYO-3. The fluorescence emission this is enhanced by intercalation within double helix. have explored saturation dsDNA bound YOYO-3 at single-molecule level studying single-pair Forster resonance energy transfer (FRET) from an donor, Alexa Fluor 488, tagged 5′ end helix acceptor, YOYO-3, to same molecule. spontaneous binding gives rise effective distribution different FRET efficiencies and, therefore, donor–acceptor (D–A) distances. These distributions reveal existence multiple states Steady-state time-resolved circular dichroism confirmed presence DNA-bound aggregate conspicuous high dye/base pair ratios. spectral features suggest that it may str...
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