A comparative evaluation of a new fully automated assay for von Willebrand factor collagen binding activity to an established method.

摘要: Introduction Laboratory diagnosis of von Willebrand disease (VWD) is made by the measurement factor (VWF) protein level and its activities. Current VWF activity tests include ristocetin cofactor collagen binding (VWF:CB) assays. Aim We have undertaken an evaluation a new fully automated VWF:CB assay relative to established enzyme-linked immunosorbent (ELISA) method. Methods The two analytical systems operate with different detection principles: chemiluminescent method performed on ACL AcuStar Analyzer (the former) colorimetric ELISA Asserachrom Stago latter) (type III from human placenta). The HemosIL 2-step immunoassay that uses magnetic particles coated type triple-helical peptide. levels were determined in 50 healthy subjects 100 VWD patients (22 1, 73 2 5 3). Results Eleven samples reported values below lower limit one or both methods. showed good correlation (r > .9, mean bias 3.85 IU/dL) samples. One 150 gave inconsistent results using assays, leading uncertain 1 (ELISA method) MCB (fully method). Conclusion The rapid simple use, ready-to-use reagent cartridges. This assay, addition VWF:Ag VWF:RCo same platform, gives additional information for nonspecialized reference laboratories.