Bioluminescence as Gold Standard for Validation of Optical Imaging Modalities in Peritoneal Carcinomatosis Animal Models

摘要: Background: The outcome of cytoreductive surgery in patients with peritoneal carcinomatosis is influenced by incomplete resection as a result inadequate detection tumor, i.e. residual disease. future perspective complete resection, made possible application intraoperative near-infrared fluorescence imaging (NIRF), led to the development and validation bioluminescent colorectal xenograft rat model act gold standard for evaluation new optical modalities. Methods: Twenty nude rats were inoculated intraperitoneally 2 × 106 luciferase-labeled human tumor cells (HT-29-luc-D6). index (PCI) was estimated using visual observation (PCI-VO) VO combined bioluminescence (PCI-BLI). Subsequently, BL images presented, tissue localized PCI-BLI scoring compared PCI-VO. Results: BLI revealed additional tissue, confirmed HE staining, alone 7 out 8 (p Conclusion: developed turned be suitable. use more sensitive alone. In this model, significantly detected disease, therefore, can denominated modalities like NIRF.