Use of a Chimeric Synthetic Peptide from the Core p19 Protein and the Envelope gp46 Glycoprotein in the Immunodiagnosis of HTLV-II Virus Infection
作者： Milenen Hernández Marin , Yadaris Márquez Bocalandro , René Vázquez Vallejo , Chryslaine Rodríguez Tanty , David Higginson Clark
DOI: 10.1081/PB-120018367
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摘要: Abstract A chimeric synthetic peptide incorporating immunodominant epitope of the p19 gag protein (116–134) and gp46 env (178–200) HTLV-II virus, separated by two glycine residues, was synthesized conventional solid-phase synthesis. The antigenic activity this evaluated Ultramicro Enzyme-linked immunosorbent assay (UMELISA) using panels anti-HTLV-II positive sera (n = 9), anti-HTLV-I/II (n = 2), HTLV-positive (untypeable) serum samples (n = 1), anti-HTLV-I (n = 14), while specificity with from healthy blood donors (n = 20). efficacy in immunoassays compared monomeric peptides. Data demonstrated that most reactive because it detected antibodies to virus efficiently. This may be related adsorption solid surface accessibility antibodies. results indicate as coatin...
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