Effects of two-hour exposure to environmental and high concentrations of methylmercury on the transcriptome of the macrophyte Elodea nuttallii.

摘要: The effects of two methylmercury (CH3Hg+, MeHg) concentrations, representative environmental level and extreme contamination, were investigated on the macrophyte Elodea nuttallii during a 2h-exposure combining transcriptomic (RNA-Seq), physiological endpoints (pigment contents, activity anti-oxidative stress enzymes) bioaccumulation. Exposure to MeHg induced up- down-regulation numerous genes (4389 16853 for 10ngL-1 10μgL-1 exposure, respectively) involved in sugar, amino acid secondary metabolism (e.g. cinnamic acid, flavonoids) at both concentrations. Genes coding photosynthesis, membrane integrity, metal homeostasis, water transport enzymes additionally down-regulated higher concentration. At level, exposure concentrations resulted strong increase anthocyanin content shoots. Chlorophyll antioxidant enzyme activities unchanged. data suggest that was able efficiently cope with resulting from possibly by using as anti-oxidant S-rich acids (such cysteine methionine) chelators. Transcriptomics analysis enabled gaining novel insights molecular primary producers, which are one main entry pathway hazardous aquatic food webs.