Two-Dimensional Algal Collection and Assembly by Combining AC-Dielectrophoresis with Fluorescence Detection for Contaminant-Induced Oxidative Stress Sensing.

摘要: An alternative current (AC) dielectrophoretic lab-on-chip setup was evaluated as a rapid tool of capture and assembly microalga Chlamydomonas reinhardtii in two-dimensional (2D) close-packed arrays. electric field 100 V·cm−1, Hz applied for 30 min found optimal to collect assemble the algae into single-layer structures closely packed cells without inducing cellular oxidative stress. Combined with stress specific staining fluorescence microscopy detection, capability using 2D whole-cell on-chip follow reactive oxygen species (ROS) production during short-term exposure several environmental contaminants, including mercury, methylmercury, copper, copper oxide nanoparticles (CuO-NPs), diuron explored. The results showed significant increase ROS when C. exposed high concentrations CuO-NPs, 10−5 M Cu. Overall, this study demonstrates potential combining AC-dielectrophoretically assembled algal cell metabolic analysis staining, analytical probing effect contaminants highly impacted environment.