Probing Contaminant-Induced Alterations in Chlorophyll Fluorescence by AC-Dielectrophoresis-Based 2D-Algal Array.

摘要: The investigation of contaminant impact on algae requires rapid and reliable cell collection optical detection. capability alternative current (AC) dielectrophoresis (DEP) whole arrays with combined fluorescence microscopy detection to follow the alterations chlorophyll during environmental exposure was explored. application an AC-field 100 V cm−1, Hz for 30 min capture immobilize cells green alga Chlamydomonas reinhardtii in two-dimensional (2D) does not induce changes fluorescence. results demonstrate that DEP-based 2D-arrays allow non-invasive change upon high concentrations copper oxide nanoparticles ionic copper. These were agreement data obtained by flow cytometry used as a comparative method. tool also applied effect number ubiquitous contaminants such inorganic mercury, methylmercury, diuron. However, statistically significant short-term observed only mercury. Overall, algal appear be suitable stain-free probing effects photosynthetic microorganisms highly polluted environment.