Immunoassays distinguishing between HNL/NGAL released in urine from kidney epithelial cells and neutrophils
作者： Johan Mårtensson , Shengyuan Xu , Max Bell , Claes-Roland Martling , Per Venge
DOI: 10.1016/J.CCA.2012.05.010
关键词:
摘要: Abstract Background The distinction between monomeric human neutrophil lipocalin/neutrophil gelatinase-associated lipocalin (HNL/NGAL), secreted by injured kidney tubular cells, and dimeric HNL/NGAL, released activated neutrophils, is important to accurately diagnose acute injury (AKI). Methods 132 urine samples from 44 intensive care unit (ICU) patients five non-ICU with urinary tract infections (UTIs) were analyzed two monoclonal enzyme-linked immunosorbent assays (ELISA-1 ELISA-2). presence of and/or HNL/NGAL in each sample was visualized Western blotting. Results ELISA-1 detected both whereas the ELISA-2 almost exclusively an area under receiver‐operating characteristics curve (AuROC) 0.90. ELISA-1/ELISA-2 ratio form AuROC 0.92. In 32 AKI patients, dimer-specific levels decreased pre-AKI monomer-specific gradually increased beyond diagnosis. High low ratios a predominance UTIs. Conclusions combination, our ELISAs distinguish produced epithelium, neutrophils during development, as well reduce confounding effect involvement when bacteriuria present.
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