Global gene expression reveals a set of new genes involved in the modification of cells during erythroid differentiation
作者： A. F. Da Cunha , A. F. Brugnerotto , A. S. Duarte , C. Lanaro , G. G. L. Costa
DOI: 10.1111/J.1365-2184.2010.00679.X
关键词:
摘要: Objectives: Erythroid differentiation is a dynamic process in which pluripotent stem cell undergoes series of developmental changes that commit it to specific lineage. These alterations involve gene expression profiles. In this study, profiles during human erythroid cells normal blood donor were evaluated using SAGE. Materials and methods: Global was collected immediately before addition erythropoietin (0 h) 192 336 h after hormone. Real-time PCR used evaluate activation differentially expressed genes. Results: The data indicate global aspects the transcriptome similar majority genes relatively small set probably involved modification differentiation. We have identified 93 development, some these confirmed by qPCR. Various including EYA3, ERH, HES6, TIMELESS TRIB3 found be homologous those Drosophila melanogaster here are described for first time development. An important unique carboxypeptidase inhibitor mammalians, LXN, also identified. Conclusions: results study amplify previously published may contribute comprehension identification new target concerning diseases.
fapesp.br
sci-hub.se 参考文章(45)
RA Shivdasani, SH Orkin, The transcriptional control of hematopoiesis. Blood. ,vol. 87, pp. 4025- 4039 ,(1996) , 10.1182/BLOOD.V87.10.4025.BLOODJOURNAL87104025
Xue Li, Kenneth A Ohgi, Jie Zhang, Anna Krones, Kevin T Bush, Christopher K Glass, Sanjay K Nigam, Aneel K Aggarwal, Richard Maas, David W Rose, Michael G Rosenfeld, None, Eya protein phosphatase activity regulates Six1–Dach–Eya transcriptional effects in mammalian organogenesis Nature. ,vol. 426, pp. 247- 254 ,(2003) , 10.1038/NATURE02083
E. Wojcik, Khoi Dang-Vu, S. I. Tsubota, A. M. Murphy, H. Fares, ENHANCER OF RUDIMENTARYP1, E(R)P1, A HIGHLY CONSERVED ENHANCER OF THE RUDIMENTARY GENE Genetics. ,vol. 138, pp. 1163- 1170 ,(1994) , 10.1093/GENETICS/138.4.1163
Alex J Bowers, Sheila Scully, John F Boylan, SKIP3, a novel Drosophila tribbles ortholog, is overexpressed in human tumors and is regulated by hypoxia Oncogene. ,vol. 22, pp. 2823- 2835 ,(2003) , 10.1038/SJ.ONC.1206367
Ronit I. Yarden, Sherly Pardo-Reoyo, Magda Sgagias, Kenneth H. Cowan, Lawrence C. Brody, BRCA1 regulates the G2/M checkpoint by activating Chk1 kinase upon DNA damage Nature Genetics. ,vol. 30, pp. 285- 289 ,(2002) , 10.1038/NG837
G. Jimenez, S. D. Griffiths, A. M. Ford, M. F. Greaves, T. Enver, Activation of the beta-globin locus control region precedes commitment to the erythroid lineage. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 10618- 10622 ,(1992) , 10.1073/PNAS.89.22.10618
E. Pogge von Strandmann, S. Senkel, G.U. Ryffel, ERH (enhancer of rudimentary homologue), a conserved factor identical between frog and human, is a transcriptional repressor. Biological Chemistry. ,vol. 382, pp. 1379- 1385 ,(2001) , 10.1515/BC.2001.170
Mark Gelsthorpe, Malini Pulumati, Carrol McCallum, Khoi Dang-Vu, Stuart I Tsubota, The putative cell cycle gene, enhancer of rudimentary, encodes a highly conserved protein found in plants and animals. Gene. ,vol. 186, pp. 189- 195 ,(1997) , 10.1016/S0378-1119(96)00701-9
C Pina, T Enver, Differential contributions of haematopoietic stem cells to foetal and adult haematopoiesis: insights from functional analysis of transcriptional regulators Oncogene. ,vol. 26, pp. 6750- 6765 ,(2007) , 10.1038/SJ.ONC.1210759
T Rzymski, A Paantjens, J Bod, A L Harris, Multiple pathways are involved in the anoxia response of SKIP3 including HuR-regulated RNA stability, NF-kappaB and ATF4. Oncogene. ,vol. 27, pp. 4532- 4543 ,(2008) , 10.1038/ONC.2008.100