Antisense DNA inhibition of tumor growth induced by c-Ha-ras oncogene in nude mice.

摘要: Antisense DNA has shown an ability to target specific oncogene transcripts and inhibit their expression in cells, but the degree which sustained treatment can suppress total levels of oncogenic product alter tumorigenesis vivo remains be determined. In this study, NIH-3T3 cells transformed by activated c-Ha-ras from T24 human bladder cancer were treated for 3 consecutive days vitro with antisense pentadecamer complementary a 5'-flanking region RNA transcript. Following treatment, portion was lysed measurement RAS p21 while remaining evaluated tumorigeneity injection s.c. into athymic nude mice at dose 5 x 10(5) cells/mouse. The anti-c-Ha-ras reduced cellular more than 90% nonspecific control approximately 20%. Tumor growth significantly up 14 following end implantation whereas had no significant effect. These effects on tumor evident two different strains both males females. It is suggested that pronounced decrease produced resulted reversal phenotype, it accounts prolonged inhibition treatment.