B cell-specific loss of histone 3 lysine 9 methylation in the V(H) locus depends on Pax5.
作者： Kristen Johnson , David L Pflugh , Duonan Yu , David G T Hesslein , Kuo-I Lin
DOI: 10.1038/NI1099
关键词:
摘要: Immunoglobulin heavy chain rearrangement (VH-to-DJH) occurs only in B cells, suggesting it is inhibited other lineages. Here we found that the mouse VH locus, methylation of lysine 9 on histone H3 (H3-K9), a mark inactive chromatin, was present non–B lineage cells but absent cells. As others have shown H3-K9 can inhibit V(D)J recombination engineered substrates, our data support idea inhibits endogenous VH-to-DJH recombination. We also show Pax5, transcription factor required for cell commitment, necessary and sufficient removal locus provide evidence one function Pax5 to remove this inhibitory modification by mechanism exchange, thus allowing cell–specific
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