Pathogen recognition receptors in channel catfish: I. Identification, phylogeny and expression of NOD-like receptors.
作者： K.V. Rajendran , Jiaren Zhang , Shikai Liu , Huseyin Kucuktas , Xiuli Wang
DOI: 10.1016/J.DCI.2011.12.005
关键词:
摘要: Innate immune system plays a significant role in all multicellular organisms. The key feature of the is its ability to recognize and respond invading microorganisms. Vertebrates including teleost fish have evolved an array pathogen recognition receptors (PRRs) for detecting responding various pathogen-associated molecular patterns (PAMPs), Toll-like (TLRs), nucleotide-binding domain, leucine-rich repeat containing (NLRs), retinoic acid inducible gene I (RIG-I) like (RLRs). In this study, we identified 22 NLRs six members NLR-A subfamily (NODs), two NLR-B subfamily, 11 NLR-C three genes that do not belong any these subfamilies: Apaf1, CIITA, NACHT-P1. Phylogenetic analysis indicated orthologs mammalian NOD1, NOD2, NOD3, NOD4, NOD5 were catfish. addition, additional truncated NOD3-like was also While identities A could be established, subfamilies inconclusive at present. Expression representative NLR analyzed using RT-PCR qRT-PCR. healthy catfish tissues, tested found ubiquitously expressed tissues. Analysis expression after bacterial infection with Edwardsiella ictaluri revealed up-regulation spleen liver, but down-regulation intestine head kidney, suggesting their involvement responses against intracellular tissue-specific manner. exhibited amazing similarity profiles infection, co-regulation genes.
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