InvasiveShigella flexneriActivates NF-κB Through a Lipopolysaccharide-Dependent Innate Intracellular Response and Leads to IL-8 Expression in Epithelial Cells
作者： Dana J. Philpott , Shoji Yamaoka , Alain Israël , Philippe J. Sansonetti
DOI: 10.4049/JIMMUNOL.165.2.903
关键词:
摘要: The pathogenesis of Shigella flexneri infection centers on the ability this organism to invade epithelial cells and initiate an intense inflammatory reaction. Because NF-kappa B is important transcriptional regulator genes involved in inflammation, we investigated role transcription factor during S. cells. Infection HeLa with invasive induced DNA-binding activity; noninvasive strains did not lead activation. pathway leading activation by kinases, B-inducing kinase, I kappa kinase-1, kinase-2. was linked because activated IL-8 promoter-driven reporter gene, site within promoter indispensable for its induction. Microinjection bacterial culture supernatants into suggested that LPS responsible infection. In conclusion, eukaryotic cells, which suggests a modulating immune response vivo.
highwire.org参考文章(68)
E. T. Rietschel, H. Brade, O. Holst, L. Brade, S. Müller-Loennies, U. Mamat, U. Zähringer, F. Beckmann, U. Seydel, K. Brandenburg, A. J. Ulmer, T. Mattern, H. Heine, J. Schletter, H. Loppnow, U. Schönbeck, H.-D. Flad, S. Hauschildt, U. F. Schade, F. Di Padova, S. Kusumoto, R. R. Schumann, Bacterial Endotoxin: Chemical Constitution, Biological Recognition, Host Response, and Immunological Detoxification Current Topics in Microbiology and Immunology. ,vol. 216, pp. 39- 81 ,(1996) , 10.1007/978-3-642-80186-0_3
Ian M. Rosenberg, Steven L. Brandwein, Paul L. Beck, Hans-Christian Reinecker, Daniel K. Podolsky, Elke Cario, Lipopolysaccharide Activates Distinct Signaling Pathways in Intestinal Epithelial Cell Lines Expressing Toll-Like Receptors The Journal of Immunology. ,vol. 164, pp. 966- 972 ,(2000) , 10.4049/JIMMUNOL.164.2.966
E Muñoz, G Courtois, P Veschambre, P Jalinot, A Israël, Tax induces nuclear translocation of NF-kappa B through dissociation of cytoplasmic complexes containing p105 or p100 but does not induce degradation of I kappa B alpha/MAD3. Journal of Virology. ,vol. 68, pp. 8035- 8044 ,(1994) , 10.1128/JVI.68.12.8035-8044.1994
D Kufe, D Spriggs, K Imamura, Expression of tumor necrosis factor receptors on human monocytes and internalization of receptor bound ligand. Journal of Immunology. ,vol. 139, pp. 2989- 2992 ,(1987)
Roy Lester Whistler, Melville Lawrence Wolfrom, Derek H. Shaw, James N. BeMiller, Methods in Carbohydrate Chemistry ,(1965)
Stanimir Vuk‐Pavlović, John S. Kovach, Recycling of tumor necrosis factor-alpha receptor in MCF-7 cells. The FASEB Journal. ,vol. 3, pp. 2633- 2640 ,(1989) , 10.1096/FASEBJ.3.14.2556313
N Mukaida, Y Mahe, K Matsushima, Cooperative interaction of nuclear factor-kappa B- and cis-regulatory enhancer binding protein-like factor binding elements in activating the interleukin-8 gene by pro-inflammatory cytokines. Journal of Biological Chemistry. ,vol. 265, pp. 21128- 21133 ,(1990) , 10.1016/S0021-9258(17)45336-1
Lars Eckmann, Hyun Chae Jung, Cornelia Schürer-Maly, Asit Panja, Ewa Morzycka-Wroblewska, Martin F. Kagnoff, Differential cytokine expression by human intestinal epithelial cell lines: Regulated expression of interleukin 8 Gastroenterology. ,vol. 105, pp. 1689- 1697 ,(1993) , 10.1016/0016-5085(93)91064-O
J. E. Galan, Li Mei Chen, S. Hobbie, R. J. Davis, Involvement of mitogen-activated protein kinase pathways in the nuclear responses and cytokine production induced by Salmonella typhimurium in cultured intestinal epithelial cells. Journal of Immunology. ,vol. 159, pp. 5550- 5559 ,(1997)
Mireille Delhase, Makio Hayakawa, Yi Chen, Michael Karin, Positive and Negative Regulation of IκB Kinase Activity Through IKKβ Subunit Phosphorylation Science. ,vol. 284, pp. 309- 313 ,(1999) , 10.1126/SCIENCE.284.5412.309