A role for heparan sulfate 3-O-sulfotransferase isoform 2 in herpes simplex virus type 1 entry and spread.

摘要: Heparan sulfate (HS) 3-O-sulfotransferase isoform-2 (3-OST-2), which belongs to a family of enzymes capable generating herpes simplex virus type-1 (HSV-1) entry and spread receptors, is predominantly expressed in human brain. Despite its unique expression pattern, the ability 3-OST-2 mediate HSV-1 cell-to-cell fusion not known. Our results demonstrate that can render Chinese hamster ovary K1 (CHO-K1) cells susceptible wild-type mutant strains HSV-1. Evidence for generation gD receptors by were suggested gD-mediated interference assay 3-OST-2-expressing CHO-K1 preferentially bind gD, could be reversed prior treatment with HS lyases (heparinases II/III). In addition, acquired fuse cells-expressing glycoproteins, phenomenon mimics way viral vivo. Demonstrating specificity, cell was inhibited soluble 3-O-sulfated forms HS, but unmodified HS. Taken together, our raise possibility role infection