Comparative analysis of integrin expression on monocyte‐derived macrophages and monocyte‐derived dendritic cells

摘要: Both macrophages (MAC) and dendritic cells (DC) are members of the mononuclear phagocyte system (MPS) with monocytes (MO) as common precursor cells. Cells MPS able to take up, process present antigens T lymphocytes, thereby inducing a primary or secondary immune response. Adhesion molecules crucial importance for interaction antigen-presenting cells, especially lymphocytes. By representational difference analysis, we identified CD49c (VLA-3), member beta1-integrin family adhesion receptors, differentiation-associated antigen in MO-derived MAC. In contrast, DC did not express mRNA. These data prompted us compare integrin expression pattern MAC DC. cell types showed low alpha-chains beta1-integrins CD49a, CD49b, CD49d CD49e, whereas marked was observed CD49f. Expression both integrins increased during MO differentiation, but detectable on parallel beta1-chain (CD29) clearly up-regulated differentiation only weakly expressed On other hand, beta2-integrins CD11a, CD11b, CD11c CD18 were all Beside their role cell-cell adhesion, also known possible binding bacteria lipopolysaccharide (LPS), high LPS concentrations. Therefore investigated response versus terms tumour necrosis factor-alpha (TNF-alpha) release. less responsive doses LPS, which can easily be explained by very CD14 compared TNF-alpha comparable when stimulated Our results show specific, differentiation-dependent beta1- beta2-integrin vitro-generated We suggest that CD11/CD18 could involved As is an activation stimulus DC, may important successful maturation initiation