作者: Shoko Iwaki , Christine Tkaczyk , Anne B. Satterthwaite , Kristina Halcomb , Michael A. Beaven
关键词:
摘要: Stem cell factor (SCF) acts in synergy with antigen to enhance the calcium signal, degranulation, activation of transcription factors, and cytokine production human mast cells. However, underlying mechanisms for this remain unclear. Here we show, utilizing bone marrow-derived cells (BMMCs) from Btk Lyn knock-out mice, that via plays a key role promoting synergy. As cells, SCF enhanced degranulation BMMCs. In Btk-/- BMMCs, which there was partial reduction capacity degranulate response antigen, unable residual antigen-mediated degranulation. Furthermore, as ability promote abrogated The impairment responses correlated an inability augment phospholipase Cγ1 mobilization, phosphorylate NFκB NFAT gene these Similar studies Lyn-/- Btk-/-/Lyn-/- BMMCs indicated regulator responses. These data demonstrate, first time, is Kit-mediated amplification pathway augments FcϵRI-mediated activation.