BUT-1: a new member in the chromosomal inducible class C β-lactamases family from a clinical isolate of Buttiauxella sp.

作者: Vincent Fihman , Martin Rottman , Yahia Benzerara , Françoise Delisle , Roger Labia

DOI: 10.1111/J.1574-6968.2002.TB11293.X

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摘要: Abstract An atypical Enterobacteriaceae strain with a β-lactam susceptibility pattern of inducible cephalosporinase was isolated in Tenon Hospital (Paris, France) from patient’s skull wound infection. Identifications by the API-50CHE biochemical system and 16S rRNA gene sequencing concluded that it member Buttiauxella genus. The bla cloned sequenced. deduced translated product 383-amino acid protein (BUT-1) 75–78% identity chromosomal AmpC β-lactamases Citrobacter freundii , Enterobacter aerogenes cloacae Escherichia coli . isoelectric point 9.0 kinetic constants BUT-1 were comparable results described for other Ambler class C enzymes. associated ampR transcriptional regulator divergently transcribed common intercistronic region, genetic organization already β-lactamases. amino sequence AmpR shared 85% 81% E. C. respectively.

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