Genetic variation in Mycobacterium tuberculosis isolates from a London outbreak associated with isoniazid resistance.

摘要: The largest outbreak of isoniazid-resistant (INH-R) Mycobacterium tuberculosis in Western Europe is centred North London, with over 400 cases diagnosed since 1995. In the current study, we evaluated genetic variation a subset clinical samples from hypothesis that these isolates have unique biological characteristics served to prolong outbreak. Fitness assays, mutation rate estimation, and whole-genome sequencing were performed test for selective advantage compensatory mutations. This detailed analysis INH-R suggests this consists successful, closely related, circulating strains heterogeneous resistance profiles little or no associated fitness cost impact on their rate. Specific deletions SNPs could be peculiar feature M. isolates, potentially explain persistence years.