Genome-Wide Analyses on Loss of Heterozygosity in Head and Neck Squamous Cell Carcinomas
作者： Levent Bekir Beder , Mehmet Gunduz , Mamoru Ouchida , Kunihiro Fukushima , Esra Gunduz
DOI: 10.1097/01.LAB.0000047489.26246.E1
关键词:
摘要: Head and neck squamous cell carcinoma (HNSCC) is a frequent malignancy with poor survival rate. Identifying the tumor suppressor gene (TSG) loci by genomic studies an important step to uncover molecular mechanisms involved in HNSCC pathogenesis. We therefore performed comprehensive analyses on loss of heterozygosity (LOH) using genome-wide panel 191 microsatellite markers 22 samples. found 53 significantly high LOH (>30%) 21 chromosomal arms; highest values those were observed 3p, 9p, 13q, 15q, 17p, corresponding D3S2432 (67%), D9S921-D9S925 (67%) GATA62F03 (86%), D13S1493 (60%), D15S211 (62%), D17S1353 (88%), respectively. Fifteen hot spots defined 13 arms: 2q22-23, 4p15.2, 4q24-25, 5q31, 8p23, 9p23-24, 9q31.3, 9q34.2, 10q21, 11q21-22.3, 14q11-13, 14q22.3, 17p13, 18q11, 19q12 as reported previously HNSCCs. Furthermore, we identified five novel three arms at 2q33 (D2S1384), 2q37 (D2S125), 8q12-13 (D8S1136), 8q24 (D8S1128), 15q21 (D15S211). In conclusion, our allelotype have unveiled confirmed total 20 possible TSG that could be development HNSCC. These results provide useful clues for identification putative TSGs fine mapping suspected regions subsequent analysis functional genes.
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